Enum: EnumSequencingStrategy

Permissible Values

Value	Meaning	Description	Additional Info
NCIT:C101294	NCIT:C101294	A procedure that can determine the DNA sequence for nearly the entire genome ...	Title: Whole Genome Sequencing
NCIT:C101295	NCIT:C101295	A procedure that can determine the DNA sequence for all of the exons in an in...	Title: Whole Exome Sequencing
NCIT:C192768	NCIT:C192768	The determination of the sequence of purines and pyrimidines in a sample cont...	Title: RNA Sequence Analysis
NCIT:C129432	NCIT:C129432	A procedure that can determine the RNA sequences for all or part of the poly-...	Title: mRNA Sequencing
NCIT:C156057	NCIT:C156057	A next-generation or massively parallel high-throughput DNA sequencing-based ...	Title: MicroRNA Sequencing
NCIT:C171152	NCIT:C171152	A procedure that can determine the nucleotide sequence for all of the RNA tra...	Title: Single Cell RNA Sequencing
NCIT:C165600	NCIT:C165600	An application of next-generation targeted sequencing where tumor-specific ge...	Title: Tumor Panel Sequencing
NCIT:C173599	NCIT:C173599	An application of next-generation targeted sequencing where a disease-specifi...	Title: Disease Panel Gene Sequencing
NCIT:C198412	NCIT:C198412	An application of next-generation targeted sequencing where the sequencing pr...	Title: Multi-gene Panel Sequencing
NCIT:C204828	NCIT:C204828	A method to enrich and sequence genomic regions of interest	Title: Targeted Capture Sequencing
NCIT:C158253	NCIT:C158253	A DNA sequencing technique in which selected regions of an individual's genom...	Title: Targeted Genome Sequencing
NCIT:C158252	NCIT:C158252	A type of whole transcriptome shotgun sequencing technique in which select po...	Title: Targeted Transcriptome Sequencing
NCIT:C204820	NCIT:C204820	A strategy to obtain DNA sequences from (all) methylated sites	Title: Methyl-binding Domain Sequencing
NCIT:C204821	NCIT:C204821	A strategy to obtain DNA sequences from methylated sites	Title: Methylated DNA Immunoprecipitation Sequencing
NCIT:C204822	NCIT:C204822	A method to study DNA methylation	Title: Methylation-Sensitive Restriction Enzyme Sequencing
NCIT:C20085	NCIT:C20085	The global analysis of cellular proteins	Title: Proteomics
NCIT:C146818	NCIT:C146818	A proprietary next-generation DNA sequencing technology from Oxford Nanopore ...	Title: Oxford Nanopore Sequencing
NCIT:C223895	NCIT:C223895	The sequencing of long, continuous DNA fragments	Title: Long Read Sequencing

Slots

Name	Description
experiment_strategy	The sequencing strategy used

Identifier and Mapping Information

Schema Source

from schema: https://includedcc.org/common-access-model

LinkML Source

name: EnumSequencingStrategy
from_schema: https://includedcc.org/common-access-model
rank: 1000
permissible_values:
  NCIT:C101294:
    text: NCIT:C101294
    description: A procedure that can determine the DNA sequence for nearly the entire
      genome of an individual.
    meaning: NCIT:C101294
    title: Whole Genome Sequencing
  NCIT:C101295:
    text: NCIT:C101295
    description: A procedure that can determine the DNA sequence for all of the exons
      in an individual.
    meaning: NCIT:C101295
    title: Whole Exome Sequencing
  NCIT:C192768:
    text: NCIT:C192768
    description: The determination of the sequence of purines and pyrimidines in a
      sample containing ribonucleic acid (RNA).
    meaning: NCIT:C192768
    title: RNA Sequence Analysis
  NCIT:C129432:
    text: NCIT:C129432
    description: A procedure that can determine the RNA sequences for all or part
      of the poly-A tail-containing messenger RNA transcripts in an individual.
    meaning: NCIT:C129432
    title: mRNA Sequencing
  NCIT:C156057:
    text: NCIT:C156057
    description: A next-generation or massively parallel high-throughput DNA sequencing-based
      procedure that can identify and quantify the microRNA sequences present in a
      biological sample.
    meaning: NCIT:C156057
    title: MicroRNA Sequencing
  NCIT:C171152:
    text: NCIT:C171152
    description: A procedure that can determine the nucleotide sequence for all of
      the RNA transcripts in an amplified nucleotide sample that was derived from
      a single cell.
    meaning: NCIT:C171152
    title: Single Cell RNA Sequencing
  NCIT:C165600:
    text: NCIT:C165600
    description: An application of next-generation targeted sequencing where tumor-specific
      genes are selected for sequencing. Using next-generation techniques, chosen
      sequences can be sequenced to high depths that facilitate the identification
      of variants that are present at low allele frequencies.
    meaning: NCIT:C165600
    title: Tumor Panel Sequencing
  NCIT:C173599:
    text: NCIT:C173599
    description: An application of next-generation targeted sequencing where a disease-specific
      group of genes or loci are assayed for sequence alterations as a screening procedure
      for the disease.
    meaning: NCIT:C173599
    title: Disease Panel Gene Sequencing
  NCIT:C198412:
    text: NCIT:C198412
    description: An application of next-generation targeted sequencing where the sequencing
      primers are designed to specifically target a panel of multiple genes or loci
      of interest.
    meaning: NCIT:C198412
    title: Multi-gene Panel Sequencing
  NCIT:C204828:
    text: NCIT:C204828
    description: A method to enrich and sequence genomic regions of interest. Sequences
      are selected by oligonucleotides that are used to pull-down complementary DNA
      through hybridization.
    meaning: NCIT:C204828
    title: Targeted Capture Sequencing
  NCIT:C158253:
    text: NCIT:C158253
    description: A DNA sequencing technique in which selected regions of an individual's
      genome are sequenced.
    meaning: NCIT:C158253
    title: Targeted Genome Sequencing
  NCIT:C158252:
    text: NCIT:C158252
    description: A type of whole transcriptome shotgun sequencing technique in which
      select portions of the transcriptome are selected for sequencing.
    meaning: NCIT:C158252
    title: Targeted Transcriptome Sequencing
  NCIT:C204820:
    text: NCIT:C204820
    description: A strategy to obtain DNA sequences from (all) methylated sites. Genomic
      DNA is randomly fragmented with ultrasonication and then methylated fragments
      are captured by a protein with high affinity for double-stranded DNA harboring
      methylated CpGs; non-methylated DNA fragments are washed away. The methylation-enriched
      fraction is then barcode tagged and sequenced.
    meaning: NCIT:C204820
    title: Methyl-binding Domain Sequencing
  NCIT:C204821:
    text: NCIT:C204821
    description: A strategy to obtain DNA sequences from methylated sites. Genomic
      DNA is randomly sheared by sonication and immunoprecipitated with a monoclonal
      antibody that specifically recognizes 5-methylcytidine. The DNA fragments are
      then isolated and sequenced.
    meaning: NCIT:C204821
    title: Methylated DNA Immunoprecipitation Sequencing
  NCIT:C204822:
    text: NCIT:C204822
    description: A method to study DNA methylation. Genomic DNA is separately digested
      with different methylation sensitive restriction enzymes and the fragments are
      used to generate a library. Deep sequencing of the library allows for accurate
      detection of methylation sites in the genome.
    meaning: NCIT:C204822
    title: Methylation-Sensitive Restriction Enzyme Sequencing
  NCIT:C20085:
    text: NCIT:C20085
    description: The global analysis of cellular proteins. Proteomics uses a combination
      of sophisticated techniques including two-dimensional (2D) gel electrophoresis,
      image analysis, mass spectrometry, amino acid sequencing, and bio-informatics
      to resolve comprehensively, to quantify, and to characterize proteins. The application
      of proteomics provides major opportunities to elucidate disease mechanisms and
      to identify new diagnostic markers and therapeutic targets.
    meaning: NCIT:C20085
    title: Proteomics
  NCIT:C146818:
    text: NCIT:C146818
    description: A proprietary next-generation DNA sequencing technology from Oxford
      Nanopore Technologies that can directly identify and sequence a DNA molecule
      as it passes through a nanopore, driven by electrophoresis.
    meaning: NCIT:C146818
    title: Oxford Nanopore Sequencing
  NCIT:C223895:
    text: NCIT:C223895
    description: The sequencing of long, continuous DNA fragments.
    meaning: NCIT:C223895
    title: Long Read Sequencing